
DBT-SAHAJ National Facility for
Single-Molecule and Super-Resolution Imaging

Learn and adopt cutting-edge Single-molecule and super-resolution imaging
technologies to address biomedical research problems

About us
The DBT-SAHAJ National Facility for Single-Molecule and Super-Resolution Imaging at IIT Hyderabad provides access and training to the cutting-edge technologies such as
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Single-molecule tracking in live cells to quantify protein dynamics and target-search mechanism
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Single-molecule FRET for quantifying protein conformational dynamics
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Single-Molecule Localization Microscopy (SMLM)/Photoactivated Localization Microscopy (PALM)/Stochastic Optical Reconstruction Microscopy (STORM)/DNA PAINT
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Single-molecule FISH for quantifying transcription dynamics
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6D imaging (x, y, z, t, multi channel, multi position) of live/fixed cells
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Confocal microscopy equivalent deconvolution microscopy
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Total Internal Reflection (TIRF) and Highly Inclined and Laminated Optical Sheet (HILO) illumination microscopy
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Modern image processing software packages for 3D reconstruction and rendering, virtual reality based image segmentation and visualization, 3D deconvolution, particle tracking etc.
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Workshops and training for these cutting-edge technologies.
This facility is open for any internal and external users of IIT Hyderabad on a payment basis. We provide services from cell culture to sample preparation, imaging, data analysis and visualization. If you are interested in adopting any of these technologies for your research, please send us an inquiry at dbtsahaj@iith.ac.in
Upcoming Events/Workshop/Seminar/Conference

India BioImaging – Train-the-Trainer (TtT) Program on Advanced Bioimaging and Image Analysis
Hosted by the DBT-SAHAJ National Facility for Single-Molecule and Super-Resolution Imaging, IIT Hyderabad
The DBT-SAHAJ National Facility for Single-Molecule and Super-Resolution Imaging (IIT Hyderabad), in collaboration with the India BioImaging (IBI) Network and Global BioImaging, is pleased to host the Train-the-Trainer (TtT) Program on Advanced Bioimaging and Image Analysis.
This internationally recognized training program is designed to strengthen the capacity of imaging scientists, microscopy core facility staff, and life science researchers by focusing on effective teaching methodologies for advanced microscopy and image analysis. Rather than concentrating solely on imaging technologies, the program equips participants with the skills required to design, organize, and deliver high-quality training courses within their own institutions.
Program Highlights
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Learner-Centered Teaching: Develop effective strategies for delivering engaging lectures, interactive discussions, and practical image analysis sessions.
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Training Future Trainers: Build the expertise needed to organize and conduct microscopy and bioimage analysis workshops at regional and national levels.
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Course Design & Management: Learn best practices for curriculum development, hands-on training, assessment, and course logistics.
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Global Networking: Interact with experts from the India BioImaging and Global BioImaging communities, fostering international collaborations and knowledge exchange.
Target Participants
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Microscopy core facility managers and staff
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Imaging scientists and microscopists
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Life science researchers involved in user training
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Early-career researchers interested in microscopy education and capacity building
This initiative supports the long-term vision of expanding high-quality bioimaging education across India by empowering participants to become effective trainers and leaders within the imaging community.
Stay tuned for registration details, program schedule, and speaker announcements.

The International Conference cum Workshop on Single-Molecule Biophysics -SiMBio2026
Dates: [Conference : 19 - 21 January, 2026 | Workshop: 22 - 23 January, 2026]
Past Events/Workshop/Seminar
Venue: Convention Center,
Indian Institute of Technology (IIT) Hyderabad,
Telangana, India
Conference website: www.simbio2026.com
Organizing Committee
Dr. Gunjan Mehta, IIT Hyderabad, India
Dr. Mahipal Ganji, IISc Banglore, India
Prof. Masahiro Ueda, Osaka University, Japan
Prof. Naoki Watanabe, Kyoto University, Japan
Dr. Farhan Anjum, IIT Hyderabad, India
In Association with
DBT-SAHAJ National Facility for Single-Molecule and Super-Resolution Microscopy at IIT Hyderabad (www.singlemolecules-iith.com).
India Bio Imaging (IBI) India, (www.indiabioimaging.org).
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Course Highlights
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Basics of digital images
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Basics of camera technologies used in microscopy
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Basic operations of ImageJ
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How to make best representative images for publication?
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Image filtering and object segmentation
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ImageJ macros
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Colocalization analysis
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Intensity measurements
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Distance measurements
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Work on your own images