Learn and adopt cutting-edge Single-molecule and super-resolution imaging
technologies to address biomedical research problems

 

The DBT-SAHAJ Facility for Single-Molecule and Super-Resolution Imaging at IIT Hyderabad provides access and training to the cutting-edge technologies such as

•  Single-molecule tracking in live cells to quantify protein dynamics and target-search mechanism

•  Single-molecule FRET for quantifying protein conformational dynamics

•  Single-Molecule Localization Microscopy (SMLM)/Photoactivated Localization Microscopy (PALM)/Stochastic Optical   Reconstruction Microscopy (STORM)/DNA PAINT

•  Single-molecule FISH for quantifying transcription dynamics

•  6D imaging (x, y, z, t, multi channel, multi position) of live/fixed cells

• Confocal microscopy equivalent deconvolution microscopy

•  Total Internal Reflection (TIRF) and Highly Inclined and Laminated Optical Sheet (HILO) illumination microscopy

•  Modern image processing software packages for 3D reconstruction, deconvolution and rendering (Imaris 11 for Core Facilities), virtual reality-based image segmentation and visualization (using syGlass software), single particle tracking (MATLAB) etc.

•  Workshops and trainings for these cutting-edge technologies.

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This facility is open for any internal and external users of IIT Hyderabad on a payment basis. We provide services from cell culture to sample preparation, imaging, data analysis and visualization. If  you are interested in adopting any of these technologies for your research, please send us an inquiry at dbtsahaj@iith.ac.in